Cat. #152596

SVCT-MI2 Cell Line

Cat. #: 152596

Unit size: 1x10^6 cells / vial

Availability: 10-12 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Enquire or request a quote

Contributor

Inventor: Joyce Taylor-Papadimitriou ; Sidney Chang

Institute: Cancer Research UK, Lincoln's Inn Fields Institute

Primary Citation: Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-6291. PMID: 19674905.

Tool Details

Target Details

Applications

Handling

Related Tools

References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Name: SVCT-MI2 Cell Line
Cancer type: Breast cancer
Cancers detailed: Breast cancer
Research fields: Cancer;Cell biology;Drug development;Genetics
Organism: Human
Gender: Female
Tissue: Breast
Disease: Cancer
Growth properties: Adherent
Conditional: No
Description: SVCT-MI2 has been established from human breast epithelial cells being transfected with simian virus 40 (SV40) - a known oncogenic DNA virus in addition to a plasmid containing the neomycin gene and the human oncogene EJ Harvey-ras. This transformed epithelial cell line shows tumourigenicity in nude mice (post-selection of G418-resistant colonies). SVCT-MI2 enables molecular analysis of SV40 in human breast cancers.
Application: Molecular analysis of SV40 in breast cancer
Cellosaurus id: CVCL_2719

Target Details

Target: Human oncogene EJ Harvey-ras

Applications

Application: Molecular analysis of SV40 in breast cancer

Handling

Format: Frozen
Growth medium: RPMI 1640 or DMEM + 2mM Glutamine + 10ug / ml insulin + 5ug / ml hydrocortisone + 10% FBS. Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA. Note: cells may pile up when confluent.
Temperature: 37° C
Atmosphere: 5% CO2
Unit size: 1x10^6 cells / vial
Shipping conditions: Dry ice

Related Tools

Related tools: SVCT Cell Line

References

Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-6291. PMID: 19674905.

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.