Cat. #161915
SK-MEL-2 Cell Line
Cat. #: 161915
Availability: 8-10 weeks
Organism: Human
Tissue: Skin
Model: Tumourigenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lloyd J. Old, Germain Trempe
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-MEL-2 Cell Line
- Cancer: Skin cancer
- Organism: Human
- Gender: Male
- Tissue: Skin
- Donor: This cell line was established in 1972 from a metastatic site on the thigh of a 60-year-old Caucasian male with malignant melanoma
- Morphology: Polygonal
- Growth properties: Adherent
- Model: Tumourigenic
- Model description: Tumourigenic in nude mice; forms malignant melanoma
- Products or characteristics of interest: Karyotype: (P6) hypodiploid to hypertetraploid with abnormalities including dicentrics, secondary constrictions and large telocentric marker. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1
- Description: SK-MEL-2 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses wildtype B-Raf and mutant N-Ras (Q61R).
- Application: 3D cell culture
- Biosafety level: 1
Target Details
- Target: Antigen expression: Blood Type A; Rh+
Applications
- Application: 3D cell culture
Handling
- Growth medium: EMEM supplemented with FBS to a final concentration of 10%
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Str profiling: D3S1358: 14,16TH01: 9D21S11: 29,30D18S51: 15,16Penta_E: 7,16D5S818: 11,12,13D13S317: 11D7S820: 11,12D16S539: 8,9CSF1PO: 10,12Penta_D: 10,15Amelogenin: X,YvWA: 16,17D8S1179: 11,12,13TPOX: 8,9FGA: 19,24D19S433: 12,13,15D2S1338: 21,24
References
- Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359
