SG01 Cell Line

The subglottic airway is an important gatekeeper that functions to prevent lower lung infection and is a crucial area in terms of inflammation (subglottic stenosis) and malignancy.

Contributors

Inventor Institute
Jason Powell ; Chris Ward Newcastle University
Cat. #: 153460
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Head and neck cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Subglottis
Model: Immortalised Line
Growth properties: Adherent
Cancer Types In Detail: Inflammation;Subglottic stenosis
Product description: The subglottic airway is an important gatekeeper that functions to prevent lower lung infection and is a crucial area in terms of inflammation (subglottic stenosis) and malignancy.
Conditional: No
Production details: The SG01 Cell Line was derived from a single patients neoplasia-free human subglottic brushing specimen. The cells were immortalised using a SV40 Cell immortalization system, utilizing a recombinant lentiviral vector. SG01 were successfully lifted onto an air-liquid interface (ALI) culture system, using the appropriate growth medium. At ALI cells demonstrated cilia coverage and mucus production. Cells demonstrate tight epithelial junctions and relevant ion channels expression, including ENaC,…
Parental cell line: Subglottic brushing specimen
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: SG01 cells were cultured on a collagen coated container in Bronchial Epithelial Cell Growth Medium (BEGM) (available from Clonetics Corporation, CC3171 (BEBM) plus additives CC4175 or BEGM Bullet Kit, CC3170). , supplemented with 100 g/mL streptomycin and 100 U/mL penicillin at 37?‚°C and 5% CO2. Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.05% trypsin/EDTA.
References:

Establishment of an immortalized human subglottic epithelial cell line

A Human in Vitro Model of the Subglottic Airway

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