Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1]

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Cat. #153465

Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1]

Cat. #: 153465

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Eye

Model: Reporter


This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.


Inventor: Jonathon Pines

Institute: University of Cambridge

Tool Details
Target Details
Related Tools

Tool Details


  • Name: Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1]
  • Alternate name: MAD2L1
  • Research fields: Cell biology
  • Parental cell: RPE1
  • Organism: Human
  • Tissue: Eye
  • Model: Reporter
  • Conditional: No
  • Description: Somatic knock-in reporter cell line with endogenous expression of Mad2 fused to the yellow fluorescent protein Venus (Venus-Mad2 fusion protein). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cell line functions as a cell cycle reporter across the metaphase-to-anaphase transition of the cell cycle. See also the Cyclin A2 and Cyclin B1 versions of these cell lines for a broader analysis of the different phases of the cell cycle. This cell line is an endogenous reporter for Mad2 levels and activity.
  • Production details: To generate the cell line recombinant adenovirus-associated virus (rAVV)-mediated gene targeting was used to fuse the yellow fluorescent protein (Venus) ORF to the 5' of exon 1 of one allele of the MAD2L1 gene. The Mad2 was tagged at its N-terminus because a GFP-Mad2 fusion protein had previously been demonstrated to be functional in Drosophila.
  • Biosafety level: 1

Target Details

  • Target: Mad2


  • Format: Frozen
  • Growth medium: F12/DMEM (Sigma: D6241) + 2mM GlutaMAX?(Invitrogen), 10% Foetal Bovine Serum (FBS) + 0.348% sodium bicarbonate; 37?‚°C; 5% CO2.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11] ; Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6] ; Ruby-Mad2 Reporter Cell Line


  • Collin et al., 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242