Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1]
• Alternate name: MAD2L1
• Research fields: Cell biology
• Parental cell: RPE1
• Organism: Human
• Tissue: Eye
• Model: Reporter
• Conditional: No
• Description: Somatic knock-in reporter cell line with endogenous expression of Mad2 fused to the yellow fluorescent protein Venus (Venus-Mad2 fusion protein). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cell line functions as a cell cycle reporter across the metaphase-to-anaphase transition of the cell cycle. See also the Cyclin A2 and Cyclin B1 versions of these cell lines for a broader analysis of the different phases of the cell cycle. This cell line is an endogenous reporter for Mad2 levels and activity.
• Production details: To generate the cell line recombinant adenovirus-associated virus (rAVV)-mediated gene targeting was used to fuse the yellow fluorescent protein (Venus) ORF to the 5' of exon 1 of one allele of the MAD2L1 gene. The Mad2 was tagged at its N-terminus because a GFP-Mad2 fusion protein had previously been demonstrated to be functional in Drosophila.
• Biosafety level: 1

Target Details

• Target: Mad2

Handling

• Format: Frozen
• Growth medium: F12/DMEM (Sigma: D6241) + 2mM GlutaMAX?(Invitrogen), 10% Foetal Bovine Serum (FBS) + 0.348% sodium bicarbonate; 37?‚°C; 5% CO2.
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

Related Tools

• Related tools: Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11] ; Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6] ; Ruby-Mad2 Reporter Cell Line

References

• Collin et al., 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242