#153248

Pr8/22 Cell Line

Cat. #153248

Pr8/22 Cell Line

Cat. #: 153248

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Mouse

Tissue: Lymphatic Tissue

Disease: Cancer

Model: Tumour line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Timothy Ward

Institute: Cancer Research UK Manchester Institute

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Pr8/22 Cell Line
  • Cancers detailed: Drug resistant
  • Research fields: Cancer;Drug development;Genetics
  • Tool sub type: Continuous
  • Parental cell: P388
  • Organism: Mouse
  • Tissue: Lymphatic Tissue
  • Disease: Cancer
  • Growth properties: Suspension
  • Model: Tumour line
  • Conditional: No
  • Description: The cell line Pr8/22 has been derived from P388, a methylcholanthrene-induced lymphoid neoplasm originating in DBA/2 mouse and converted to ascitic form in the first mouse transfer. Pr8/22 is resistant to daunorubicin and is multi drug resistant (MDR). Cells should be treated with the drug at least once a month and before freezing, however after resuscitating daunorubicin should not be added until the first passage. It is advisable to keep a backup culture which is not drug-challenged.
  • Production details: The cell line Pr8/22 has been derived from P388, a methylcholanthrene-induced lymphoid neoplasm.
  • Cellosaurus id: CVCL_4276

Handling

  • Format: Frozen
  • Growth medium: Sub-culture routine: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37?‚°C. It is recommended to culture the cells without drug upon resuscitation until the first passage.Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS); treat once a month with 0.1?„?žM Daunorubicin.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • McGown et al. 1983. Cancer Chemother Pharmacol. 11(2):113-6. PMID: 6627597.
  • Comparative studies of the uptake of daunorubicin in sensitive and resistant P388 cell lines by flow cytometry and biochemical extraction procedures.