Cat. #161577
PaTu 8902 SMAD4 KOrec reconstituted cell line
Cat. #: 161577
Availability: 8-10 weeks
Organism: Human
Tissue: Pancreas
Disease: Cancer
Model: Transgeneic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ji?? Neu?il
Institute: Institute of Biotechnology of the Academy of Sciences of the Czech Republic
Primary Citation: Ezrova et al. Oncogene. 2021 Apr, 40(14):2539-2552. PMID: 33686239
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: PaTu 8902 SMAD4 KOrec reconstituted cell line
- Alternate name: PaTu 8902 SMAD4-deficient cells, reconstituted wt SMAD4
- Cancer: Pancreatic cancer
- Cancers detailed: Pancreatic ductal adenocarcinoma
- Research fields: Cancer
- Parental cell: PaTu 8902
- Organism: Human
- Gender: Female
- Tissue: Pancreas
- Disease: Cancer
- Morphology: Epithelial cells
- Growth properties: Adherent
- Model: Transgeneic
- Model description: SMAD4 knock-out cells, wild-type SMAD4 reconstituted using lentiviral transduction (pCDH-CMV vector)
- Crispr: Yes
- Conditional: No
- Products or characteristics of interest: Stable expression of SMAD4
- Description: SMAD4 is an important tumor suppressor involved in transforming growth factor ? (TGF?) signaling, and associated with altered mitochondrial activity. The resistance of SMAD4-deficient cells is mediated by increased mitophagic flux driven by MAPK/ERK signaling, whereas TGF?-induced resistance is autophagy-independent and linked to epithelial-to-mesenchymal transition (EMT). Mitochondria-targeted tamoxifen, a complex I inhibitor under clinical trial, overcomes resistance mediated by SMAD4-deficiency or TGF? signaling. Our data point to differential mechanisms underlying the resistance to treatment in PDAC arising from TGF? signaling and SMAD4 loss, respectively.
- Biosafety level: 1
- Recommended controls: PaTu 8902 SMAD4 KO
Handling
- Growth medium: DMEM+10% FBS
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry Ice
- Storage medium: Complete medium + 10% DMSO
- Storage conditions: Liquid Nitrogen
- Subculture routine: Trypsin-EDTA
- Cultured in antibiotics: Yes
- Mycoplasma free: Yes
Related Tools
- Related tools: PaTu 8902 SMAD4 KO cell line
References
- Ezrova et al. Oncogene. 2021 Apr, 40(14):2539-2552. PMID: 33686240
