Melan-si3 cell line
Organism: Mouse
Tissue: Skin
Disease: Non-cancer
Model: Immortalised non-cancerous cell lines
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Elena Sviderskaya
Institute: St. George's University London
Primary Citation: Theos A, et al. Mol Biol Cell. 2006 Aug;17(8):3598-612. PMID: 16760433
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Melan-si3 cell line
- Cancer: N/A
- Research fields: Tissue-specific biology
- Organism: Mouse
- Gender: Unspecified
- Tissue: Skin
- Disease: Non-cancer
- Morphology: Small, oval-fusiform
- Growth properties: Adherent
- Model: Immortalised non-cancerous cell lines
- Crispr: No
- Conditional: No
- Description: The melan-si3, -si-4 and -si5 cell lines are immortal melanocytes derived from Sisi/Sisi, Ink4a-Arf -/- C57BL/6J mice. The silver locus (si) encodes Pmel, a pigment-cell specific protein that forms functional amyloid within melanosomes to enable melanin deposition. These melanocytes retain pigmentation, but melanosomes do not contain Pmel fibrils, thus melanosomes are generally circular in shape. These cells may be useful for the study of early melanosome biogenesis.
- Application: Melanocyte cell biology
- Biosafety level: BSL1
Applications
- Application: Melanocyte cell biology
Handling
- Growth medium: RPMI + FBS (10%) + TPA (200 nM) + 2ME (100 µM) + phenylthiourea (PTU) (200 µM)
- Storage conditions: liquid N2
References
- Theos A, et al. Mol Biol Cell. 2006 Aug
- 17(8):3598-612. PMID: 16760434
