Cat. #161995
Melan-Ink4a-Arf4 cell line
Cat. #: 161995
Organism: Mouse
Tissue: Skin
Disease: Non-cancer
Model: Immortalised non-cancerous cell lines
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Elena Sviderskaya
Institute: St. George's University London
Primary Citation: Sviderskaya EV, et al. J Natl Cancer Inst. 2002 Mar 20;94(6):446-54. PMID: 11904317
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Melan-Ink4a-Arf4 cell line
- Organism: Mouse
- Tissue: Skin
- Disease: Non-cancer
- Morphology: Small, oval-fusiform
- Model: Immortalised non-cancerous cell lines
- Description: The melan-Ink4a-Arf1, -2, -3 and -4 cell lines are immortal melanocyte cell lines derived from the skin of neonatal Ink4a-Arf–/– C57BL/6J mice. These cells are visibly pigmented and display morphology typical of normal melanocytes. The INK4A-ARF locus encodes two inhibitors of cellular growth (p16INK4A and ARF) and is often mutated in a range of human cancers including melanoma. Therefore, these melanocytes can be used as a model of normal skin melanocytes in studies focussing on skin cancers and melanomas. They are also a useful tool for studies of pigmentation genetics and as a control for numerous other lines in the collection, as many of these have also been derived from mice that are null for Ink4a-Arf.
- Application: Melanocyte cell biology
Applications
- Application: Melanocyte cell biology
Handling
- Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
- Storage conditions: liquid N2
References
- Sviderskaya EV, et al. J Natl Cancer Inst. 2002 Mar 20
- 94(6):446-54. PMID: 11904317
