#151021

Anti-CyclinB1 [V152]

Cat. #151021

Anti-CyclinB1 [V152]

Cat. #: 151021

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Cyclin B1

Class: Monoclonal

Application: FACS ; IHC ; WB

Reactivity: Mammalian

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Julian Gannon

Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-CyclinB1 [V152]
  • Alternate name: CCNB1; Cyclin B1; G2/Mitotic-Specific Cyclin B1; CCNB
  • Research fields: Cancer;Cell biology;Genetics
  • Clone: V152
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: Mammalian
  • Host: Mouse
  • Application: FACS ; IHC ; WB
  • Description: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin B1 is a marker of cell proliferation and a key component of the cell cycle progression machinery. Cyclin B1 may prove to be a prognostic marker in neoplasia.
  • Immunogen: His tagged Hamster Cyclin B1 expressed in bacteria, harvested from inclusion bodies, extracted with 6M guanidine HC1 and purified on Nickel beads.
  • Isotype: IgG1
  • Myeloma used: Sp2/0-Ag14

Target Details

  • Target: Cyclin B1
  • Target background: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin B1 is a marker of cell proliferation and a key component of the cell cycle progression machinery. Cyclin B1 may prove to be a prognostic marker in neoplasia.

Applications

  • Application: FACS ; IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Gurden et al. 2010. J Cell Sci. 123(Pt 3):321-30. PMID: 20053638.
  • Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F.
  • Geley et al. 2001. J Cell Biol. 153(1):137-48. PMID: 11285280.
  • Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
  • Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
  • Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
  • Brandeis et al. 1998. Proc Natl Acad Sci U S A. 95(8):4344-9. PMID: 9539739.
  • Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero.