The MEF UNG KO Cell Line is a tool for in vivo study of the function of UNG and the effects of DNA mismatch during DNA synthesis. UNG encodes one of several uracil-DNA glycosylases.
| Inventor | Institute |
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| Thomas Lindahl | Cancer Research UK, London Research Institute: Clare Hall Laboratories |
| SKU: | 151559 |
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| Product description: | The MEF UNG KO Cell Line is a tool for in vivo study of the function of UNG and the effects of DNA mismatch during DNA synthesis. UNG encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. |
| Production details: | Primary MEFs were prepared from embryos, and permanent cell lines established from transformed clones arising spontaneously after repeated passage in culture, by standard procedures |
| Disease: | Cancer |
| Cat. #: | 151559 |
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| Provenance and Ownership: | The MEF UNG KO Cell Line is owned by Cancer Research UK. CancerTools supplies the MEF UNG KO Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing MEF UNG KO Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Blood cancer |
| Research Fields: | Genetics |
| Organism: | Mouse |
| Tissue: | Embryo |
| Model: | Knock-Out |
| Cancer Types In Detail: | B cell lymphoma |
| Format: | Frozen |
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| Storage conditions: | Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability. |
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM:F12 (1:1) + 10% FCS. |
| References: |
Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097. Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas. |
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