#154151

MDA-MB-231 TetOn-3G ROCK2 cell line

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Cat. #154151

MDA-MB-231 TetOn-3G ROCK2 cell line

Cat. #: 154151

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Transgenic

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Heather Mckinnon ; Justin Bower ; Daniel Croft

Institute: Cancer Research UK, Glasgow: The Beatson Institute

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MDA-MB-231 TetOn-3G ROCK2 cell line
  • Alternate name: Rho-associated protein kinase 2, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, ROCK-II, p164 ROCK-2
  • Cancer: Breast cancer
  • Cancers detailed: Triple negative breast (TNBC)
  • Research fields: Cancer;Cell biology;Drug development;Genetics
  • Tool sub type: Continuous
  • Parental cell: MDA-MB-231 D3H2LN-Luc cell line
  • Organism: Human
  • Tissue: Breast
  • Disease: Cancer
  • Growth properties: Adherent cell line. Membrane blebbing/cytoskeleton reorganisation and migration induced in the presence of doxycycline.
  • Model: Transgenic
  • Conditional: Yes
  • Conditional description: MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G regulated expression of ROCK2. Therefore ROCK2 expression is induced in the presence of doxycycline.
  • Description: This cell line has been engineered to enable tetracycline inducible expression of the kinase ROCK2. ROCK2 is a serine/threonine kinase which acts as a key regulator of the actin-myosin cytoskeleton and cell polarity. ROCK2 promotes contractile force generation and therefore is a modulator of cell motility and tumour cell invasion. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when impla...
  • Biosafety level: 1
  • Additional notes: Tet-On System owned by TET Systems GmbH & Co. KG.
  • Recommended controls: MDA-MB-231 D3H2LN-Luc parental line

Target Details

  • Target: ROCK2

Handling

  • Format: Frozen
  • Growth medium: 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.
  • A novel small-molecule MRCK inhibitor blocks cancer cell invasion.