MDA-MB-231 BRE-Luc/TK Renilla cell line

The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase.

Contributors

Inventor Institute
Caroline Hill The Francis Crick Institute
Cat. #: 156439
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Cell signaling and signal transduction;Developmental biology
Organism: Human
Tissue: Breast
Model: Reporter
Cancer Types In Detail: Breast cancer
Product description: The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control. BMP is a transforming growth factor ? (TGF-?) superfamily member.
Conditional description: Luciferase is expressed in the presence of bone morphogenetic protein (BMP) signalling
Production details: Made by transfecting the breast cancer cell line MDA-MB-31 with BRE-Luciferase plasmid, TK Renilla plasmid and pRetroSuper for puromycin resistance. Clonal
Parental cell line: MDA-MB-231 cell line
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 ?g/ml blasticidin
Recommended controls: MDA-MB-231 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Grnroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.

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