#157969

MCF7 ZsGreen-ZNF703 Overexpressing Cell Line

Cat. #157969

MCF7 ZsGreen-ZNF703 Overexpressing Cell Line

Cat. #: 157969

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Knock-In

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Carlos Caldas

Institute: University of Cambridge

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: MCF7 ZsGreen-ZNF703 Overexpressing Cell Line
  • Research fields: Cancer;Cell signaling and signal transduction
  • Tool sub type: Continuous
  • Parental cell: MCF7
  • Organism: Human
  • Tissue: Breast
  • Disease: Cancer
  • Model: Knock-In
  • Conditional: No
  • Description: This is a derivative of the MCF7 breast cancer cell line engineered to overexpress Zinc Finger Protein 703 (ZNF703). ZNF703 is a common Luminal B breast cancer oncogene that differentially regulates luminal and basal progenitors in human mammary epithelium. Manipulation of ZNF703 expression in the luminal MCF7 cell line modified the effects of TGF? on proliferation. ZNF703 drives the exprsesion of the green fluorescent protein ZsGreen. Please note there is a MCF7 Lenti-control cell line available to use as a negative control with this cell line (see Related tab).
  • Biosafety level: 1
  • Recommended controls: MCF7 parental line

Target Details

  • Target: Zinc Finger Protein 703 (ZNF703) (UniProt ID: Q9H7S9)

Applications

  • Application notes: The pHIV-Zsgreen plasmid was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox-WPRE content of the pSICO plasmid (Ventura et al., 2004). ZsGreen is used as a reporter for ZNF703.

Handling

  • Format: Frozen
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: MCF7 ZsGreen-ZNF703 Lenti-control Cell Line

References

  • Holland et al. 2011. EMBO Mol Med. 3(3):167-80. PMID: 21337521.