MCF7/ExeR-1 Cell Line

The MCF7/ExeR-1 Cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors.Third generation aromatase inhibitors (AIs) have proven to be effective treatment for estrogen receptor positive (ER+) breast cancer and are today recommended as first line endocrine therapy for postmenopausal ER+ breast cancer patients, making up the majority of breast cancer patients.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society, Denmark
Cat. #: 152555
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Breast
Gender: Female
Growth properties: Adherent: breast cancer cell line resistant to the aromatase inhibitor exemestane. Estrogen receptor positive. Progesterone receptor positive when grown in medium without exemestane.
Primary citation: Hole et al. 2015. Int J Oncol. 46(4):1481-1490. PMID: 25625755.
Alternate name: MCF-7/ExeR-1; ExeR-1
Product description: MCF7/ExeR-1 is a cell culture model mimicking acquired resistance of aromatase inhibitors (AIs) – an anti-cancer therapy. This breast cancer cell line was established from MCF7 cells. The cellular classification is epithelial, and their shape is polygonal.The MCF7/ExeR-1 cell line is resistant to the third generation AI – exemestane (Aromasin). Third generation AIs have proven to be effective treatment for oestrogen receptor positive (ER+) breast cancer and as such are recommended as first line endocrine therapy for postmenopausal ER+ breast cancer patients. However, a major problem is development of resistance against AIs. Previous applications of this cell line include western blot analysis of protein expression. Since molecular mechanisms of AI resistance are largely undisclosed, the development of cell lines resistant to the non-steroidal AI exemestane allows the study of the molecular basis for AI resistance to find new targets for treatment.
Gender: Female
CRISPR: Yes
Conditional: No
Production details: Exemestane-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10-7 M testosterone. A culture of MCF-7 cells were treated with 10-7 M exemetane for one week, trypsinised and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with exemestane. After ~2-3 months, the isolated colonies gave rise to exemestane-resistant cell lines, which could be grown in exemestane-containing medium with a weekly split ratio of ~1:25. The MCF-7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF-7 cell line.
Cellosaurus ID: CVCL_5A13
Parental cell line: MCF7
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum, 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 0.1 uM exemestane.
Temperature: 37° C
Atmosphere: Humidified air with 5% CO2
Mycoplasma free: Yes
References:

Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-726. PMID: 25667100.

Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755.

Images

View Gallery
MCF7 ExeR-1 Cell Line. Late log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)
MCF7 ExeR-1 Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.

Related Tools for Cell lines

Cat. # Tool Name
151014 MCF7 AREc32 Cell Line Key Info View Tool
151484 2fTGH Cell Line Key Info View Tool
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool
151626 A375P beta6-puro Cell Line Key Info View Tool
151448 CMT 167 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.