Cat. #160659
Cell wall synthesis protein KRE9-producing Pichia pastoris cell line
Cat. #: 160659
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Yeast
Model: Over-expressing
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Andoni Ramirez Garcia
Institute: University of the Basque Country (EHU)
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Cell wall synthesis protein KRE9-producing Pichia pastoris cell line
- Parental cell: Pichia pastoris cells
- Organism: Yeast
- Model: Over-expressing
- Production details: For expression of recombinant protein: P. pastoris cells containing the gene of interest were plated in YPD plus ZeocinTM agar plates and single colonies were grown in 25 ml of Buffered Glycerol-complex Medium (BMGY; 1% yeast extract, 2% peptone, 100 mM PBS (pH 6), 1.34% yeast nitrogen base (YNB), 4 x 10-5% biotin and 1% glycerol) at 30ÄÂ?°C and 250 rpm until OD600=2-6 was reached, for about 16-18 h. The pellet was collected by centrifugation and diluted to OD600=1 in Buffered Methanol-co...
Target Details
- Target: KRE9
Handling
- Format: Frozen
- Growth medium: YPD broth containing 100 ÄÂÄ?g/ml ZeocinTM at 28-30ÄÂ?°C and 250 rpm of shaking
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
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