Jurkat Zip6KO

Cell line used to study how the absence of the Zip6 transporter affects lymphocyte physiology

Contributors

Inventor Institute
Rub?Šn Vicente Universitat Pompeu Fabra
Cat. #: 161686
Cancer types: Blood cancer
Research Fields: Cancer;Immunology
Organism: Human
Tissue: PBMC
Model: Zip6KO
Growth properties: Impaired Activation
Primary citation: Colomar-Carando et al. 2019. J Immunol. 202(2):441-450. PMID: 30552163.
Product description: Cell line used to study how the absence of the Zip6 transporter affects lymphocyte physiology. Zip6 Knockout (KO) line, Zip6 is strongly upregulated early during activiation of T lymphocytes stimulated using anti-CD3/anti-CD28
CRISPR: Yes
Production details: Cells were maintained in RPMI 1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin. Where indicated, FBS was incubated according to the manufacturer’s instructions with Chelex 100 resin (Bio-Rad Laboratories) to generate Zn2+-free growth medium. Various amounts of ZnSO4 were added to the final medium to generate specific Zn2+ concentration conditions. Cells were grown at 37°C in a humidified 5% CO2 atmosphere. 
Parental cell line: Jurkat
Model description: CRISPR/Cas9–expressing Jurkat cell line used to generate a Zip6KO Jurkat cell line
Shipping conditions: Dry Ice
Growth medium: RPMI 1640 medium
Temperature: 37° C
Atmosphere: 5% CO2
References:

Colomar-Carando et al. 2019. J Immunol. 202(2):441-450. PMID: 30552163.

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