#161686

Jurkat Zip6KO

Cat. #161686

Jurkat Zip6KO

Cat. #: 161686

Organism: Human

Tissue: PBMC

Model: Zip6KO

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Rub?Šn Vicente

Institute: Universitat Pompeu Fabra

Primary Citation: Colomar-Carando et al. 2019. J Immunol. 202(2):441-450. PMID: 30552163.

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Jurkat Zip6KO
  • Parental cell: Jurkat
  • Organism: Human
  • Tissue: PBMC
  • Growth properties: Impaired Activation
  • Model: Zip6KO
  • Model description: CRISPR/Cas9–expressing Jurkat cell line used to generate a Zip6KO Jurkat cell line
  • Crispr: Yes
  • Description: Cell line used to study how the absence of the Zip6 transporter affects lymphocyte physiology.
    Zip6 Knockout (KO) line, Zip6 is strongly upregulated early during activiation of T lymphocytes stimulated using anti-CD3/anti-CD28
  • Production details: Cells were maintained in RPMI 1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin. Where indicated, FBS was incubated according to the manufacturer’s instructions with Chelex 100 resin (Bio-Rad Laboratories) to generate Zn2+-free growth medium. Various amounts of ZnSO4 were added to the final medium to generate specific Zn2+ concentration conditions. Cells were grown at 37°C in a humidified 5% CO2 atmosphere. 

Handling

  • Growth medium: RPMI 1640 medium
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Shipping conditions: Dry Ice
  • Cultured in antibiotics: 1% Penicillin-Streptomycin

References

  • Colomar-Carando et al. 2019. J Immunol. 202(2):441-450. PMID: 30552163.