#153735

Human AGS NOD1 KD (AGS cl.1) Cell Line

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Cat. #153735

Human AGS NOD1 KD (AGS cl.1) Cell Line

Cat. #: 153735

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Gastric

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Richard Ferrero

Institute: Hudson Institute of Medical Research

Tool Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Human AGS NOD1 KD (AGS cl.1) Cell Line
  • Alternate name: Human nucleotide-binding oligomerization domain-containing protein 1, NOD1
  • Cancer: Digestive/Gastrointestinal cancer
  • Cancers detailed: Gastric Adenocarcinoma;Helicobacter pylori infection
  • Research fields: Cancer;Cell signaling and signal transduction;Immunology
  • Tool sub type: Continuous
  • Parental cell: AGS
  • Organism: Human
  • Tissue: Gastric
  • Disease: Cancer
  • Description: NOD1 regulates host epithelial cell responses. Upregulates antimicrobial peptides of the beta-defensin (hBD) family secreted by epithelial cells. NOD1 is an intracellular pattern recognition receptor that mediates innate and adaptive immunity. NOD1 specifically recognises molecules with a D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety, such as the Gram-negative bacterial cell wall component, peptidoglycan, which results in the activation of the NF-kB pathway and induction of cytokines via RIPK2 and IKK-gamma. Confers reactivity to bacterial liposaccharides (LPS) and confers immunity to viral and bacterial pathogens. These cell lines have for example been used to study NOD1 responds to Helicobacter pylori infection. H. pylori causes chronic inflammation, a key precursor to gastric adenocarcinoma. AGS cells deficient in NOD1 can be used to model gastric adenocarcinoma in the context of various pathogens.
  • Production details: Human AGS NOD1 Knock-down (KD) cells were generated by integration of an expression vector containing a small interference RNA (siRNA) directed to the gene encoding the CARD domain of the NOD1 gene. Recommended Freezing Composition: 90% (v/v) FCS - 10% (v/v) DMSO
  • Biosafety level: 1
  • Recommended controls: Human AGS Control (clone siEGFP)

Handling

  • Format: Frozen
  • Growth medium: RPMI with: 10% foetal calf serum; 1% penicillin-streptomycin; and 1% L-glutamine. Subculture cells every 2-3 days in ratios of 1:2-1:4 in T25 or T75 flasks.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage medium: 90% (v/v) FCS - 10% (v/v) DMSO
  • Mycoplasma free: Yes

Related Tools

  • Related tools: Human AGS NOD1 KO (Clone 41H8) Cell Line ; Human AGS NOD1 KO (Clone 41A8) Cell Line

References

  • Grubman et al. 2010. Cell Microbiol. 12(5):626-39. PMID: 20039881.
  • The innate immune molecule, NOD1, regulates direct killing of Helicobacter pylori by antimicrobial peptides.