Cat. #154463
HPV-16-HCK cell line
Cat. #: 154463
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Organism: Human
Tissue: Cervix
Disease: Cancer
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Aloysius Klingelhutz
Institute: The University of Iowa
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: HPV-16-HCK cell line
- Research fields: Cancer
- Tool sub type: Continuous
- Parental cell: Human Cervical Keratinocytes
- Organism: Human
- Tissue: Cervix
- Disease: Cancer
- Growth properties: The HPV-16-containing clones became immortal without a crisis and, at later passage, exhibited elevated levels of telomerase and higher levels of hTERT without any apparent increase in HPV-16 copy number, E6 transcript levels, or ability to degrade p53.
- Model: Immortalised Line
- Conditional: No
- Description: Contains full length HPV16 in episomal form
- Production details: The 7905 base pair (bp) clone pEFHPV-16W12E (gift from Dr. Paul F. Lambert, University of Wisconsin Medical School, Madison, WI) derived from an HPV-positive patient was utilized as the HPV-16 genome for our replication assays (Flores et al., 1999). One hundred micrograms of HPV-16 genomes was digested overnight in a 750- l reaction from the pUC19 vector using the restriction enzyme BamHI followed by heat inactivation of the enzyme. The entire digested DNA was then re-ligated in a large volum...
- Biosafety level: 1
Target Details
- Target: Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies
Handling
- Format: Frozen
- Growth medium: G418 selection (Have with and without feeders)
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- Sprague et al. 2002. Virology. 301(2):247-54. PMID: 12359427.
