HPV-16-HCK cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: HPV-16-HCK cell line
• Research fields: Cancer
• Tool sub type: Continuous
• Parental cell: Human Cervical Keratinocytes
• Organism: Human
• Tissue: Cervix
• Disease: Cancer
• Growth properties: The HPV-16-containing clones became immortal without a crisis and, at later passage, exhibited elevated levels of telomerase and higher levels of hTERT without any apparent increase in HPV-16 copy number, E6 transcript levels, or ability to degrade p53.
• Model: Immortalised Line
• Conditional: No
• Description: Contains full length HPV16 in episomal form
• Production details: The 7905 base pair (bp) clone pEFHPV-16W12E (gift from Dr. Paul F. Lambert, University of Wisconsin Medical School, Madison, WI) derived from an HPV-positive patient was utilized as the HPV-16 genome for our replication assays (Flores et al., 1999). One hundred micrograms of HPV-16 genomes was digested overnight in a 750- l reaction from the pUC19 vector using the restriction enzyme BamHI followed by heat inactivation of the enzyme. The entire digested DNA was then re-ligated in a large volum...
• Biosafety level: 1

Target Details

• Target: Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies

Handling

• Format: Frozen
• Growth medium: G418 selection (Have with and without feeders)
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

References

• Sprague et al. 2002. Virology. 301(2):247-54. PMID: 12359427.