Cat. #160841
HeLa Flp-In SLC25A6
Cat. #: 160841
Unit size: 1x10^6 cells / vial
Model: Knock-Out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Matthias Hentze
Institute: European Molecular Biology Laboratory (EMBL)
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: HeLa Flp-In SLC25A6
- Research fields: Metabolism
- Parental cell: HeLa FLP-IN T-REx
- Model: Knock-Out
- Description: To identify genes whose expression is controlled by YBX3
- Application: YBX3
- Production details: Adherent HeLa cells were grown at 37ÄÂ?°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO). Cells were grown until 70%ÄË?Â?Â80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturerÄË?Â?Âs recommendations (Lipofectamine RNAiMax, Invitrogen, cat# 137780755). YBX3 and control siRNAs were obtained from...
Target Details
- Target: YBX3
Applications
- Application: YBX3
Handling
- Format: Frozen
- Growth medium: Adherent HeLa cells were grown at 37ÄÂ?°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO) Grown in medium containing blasticidine (5mg/ml) and hygromycin (200mg/ml). Cells were grown until 70%ÄË?Â?Â80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturerÄË?Â?Âs recommendations (Lipofectamine RNAi...
- Unit size: 1x10^6 cells / vial
Related Tools
- Related tools: HeLa Flp-In SLC7A5 ; HeLa Flp-In YBX3: GFP ; HeLa Flp-In SLC7A5+SLC7A5 3ÄË?Â? UTR ; HeLa Flp-In SLC25A6+SLC7A5 3ÄË?Â? UTR
References
- The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance
