#153208

HCT 116-I6 Invasive Cell Line

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Cat. #153208

HCT 116-I6 Invasive Cell Line

Cat. #: 153208

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Colon

Disease: Cancer

Model: Cancer Model

£575.00

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Contributor

Inventor: Sandra Van Schaeybroeck

Institute: Queen's University Belfast

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: HCT 116-I6 Invasive Cell Line
  • Cancer: Digestive/Gastrointestinal cancer
  • Cancers detailed: Colorectal
  • Research fields: Cancer;Cell biology;Drug development
  • Tool sub type: Continuous
  • Parental cell: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Disease: Cancer
  • Growth properties: Invasion, migration
  • Model: Cancer Model
  • Conditional: No
  • Description: The HCT 116-I6 cell line is a sub-line of HCT 116 that demonstrates a 4- and 20-fold increase in migration and invasion rate respectively compared to the parental cell line.
  • Production details: HCT 116 cells were incubated in a Matrigel Invasion Chambers (MIC) for 72-96h. The cells that invaded through to the bottom chamber were collected and designated as â?‚€?‚˜Invasive 1â?‚€?‚™ (I1). These cells were propagated and repeatedly passed through the MIC until highly invasive HCT116-I6 cells were selected.
  • Cellosaurus id: CVCL_HG06

Handling

  • Format: Frozen
  • Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684.
  • EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer.
  • Dunne et al. 2014. Clin Cancer Res. 20(1):164-75. PMID: 24170546.
  • AXL is a key regulator of inherent and chemotherapy-induced invasion and predicts a poor clinical outcome in early-stage colon cancer.