GFP-KSC4 Keratinocyte Stem Cell Line

The GFP-KSC4 Keratinocyte Stem Cell line can be used for the generation of in vitro epidermal ‘skin equivalents’ to be used as screening tools to look at the activity of NCE’s and/or NBE’s of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments. Murine keratinocyte stem cell line.

Contributors

Inventor Institute
Julia Reichelt Newcastle University
Cat. #: 151713
Unit size: 1×10^6 cells / vial
Research Fields: Drug development;Stem cell biology
Organism: Mouse
Model: Transgenic
Growth properties: Adherent
Product description:  The GFP-KSC4 Keratinocyte Stem Cell line can be used for the generation of in vitro epidermal ‘skin equivalents’ to be used as screening tools to look at the activity of NCE’s and/or NBE’s of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments. Murine keratinocyte stem cell line.
Production details: Keratinocytes were isolated from neonatal homozygous C57BL/6-Tg(CAG-EGFP)1Osb/J mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3-J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells.
Cellosaurus ID: CVCL_W142
Disease: Dermatology
Format: Frozen
Shipping conditions: Dry ice
Growth medium: See remarks…
References:

Reichelt and Haase. 2010. Methods Mol Biol 585:59-69

PMID: 19907996 Okabe et al. 1997 FEBS Letters 407:313-9. PMID: 9175875 HĂ?›her T et al., 2011. Stem Cell Rev. PMID: 21874280 Vollmers A et al., 2011. PMID: 21892602

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