CCRF-CEM-R5 Cell line

4-HPR is a synthetic retinoid with potent pro-apoptotic activity against several types of cancer, including leukemia cells.

Contributors

Inventor Institute
Aintzane Asumendi ; Aintzane Apraiz ; Dolores Boyano University of the Basque Country (EHU)
Cat. #: 156470
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Blood cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Bone Marrow
Model: Tumour line
Growth properties: Suspension
Cancer Types In Detail: Acute Lymphoblastic Leukemia;Cancer
Product description: CCRF-CEM-R5 cell line is resistant to 4-HPR, a synthetic retinoid with potent pro-apoptotic activity against several types of cancer, including leukemia cells.
Conditional: No
Production details: CCRF-CEM-R5 cell line, resistant to up to 5 mM of 4-HPR, was obtained by gradual selection. Parental CCRF-CEM cells were continuously exposed to increasing 4-HPR concentrations, starting from 0.5 mM.
Parental cell line: CCRF-CEM
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells should be grown in RPMI 1640 with 2mM L-glutamine supplemented with 10% heat inactivated FBS. Keep at 37?‚°C in a humidified incubator containing 5% CO2. In order to preserve their resistant intermitent exposures to 5mM 4-HPR is recomended. 4-HPR removal from R5 cell culture for up to 8 passages or 3 weeks has not shown any decrease on drug resistance although it does affect endogenous sphingolipid profile (specified in the reference paper).
Recommended controls: Control cells are CCRF-CEM parental cells that are sensitive to higher than 1mM 4HPR
References:

Apraiz et al. 2011. BMC Cancer. 11:477. PMID: 22061047.

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