Catalytically-inactive-JMJD6 U2OS cell line

Cat. #161710

Catalytically-inactive-JMJD6 U2OS cell line

Cat. #: 161710

Sub-type: Continuous

Organism: Human

Tissue: Bone

Disease: Myasthenic Syndrome

Model: Knock-Out; Knock-In


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Inventor: Yvan Canitrot

Institute: Toulouse Tech Transfer

Primary Citation: Fages et al. 2020. PLoS Genet 16(6):e e1008511. PMID: 32598339.

Tool Details
Target Details
Related Tools

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Catalytically-inactive-JMJD6 U2OS cell line
  • Alternate name: JMJD6 KO+Mut
  • Cancer: Sarcoma
  • Research fields: Cancer
  • Tool sub type: Continuous
  • Parental cell: U2OS
  • Organism: Human
  • Gender: Female
  • Tissue: Bone
  • Disease: Myasthenic Syndrome
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model: Knock-Out; Knock-In
  • Model description: JMJD6 knock out using CRISPR-Cas9 and knock in of a catalytically inactive form of JMJD6
  • Crispr: Yes
  • Conditional: No
  • Receptors of note: Insulin like growth factor
  • Description: Catalitically inactive JMJD6 U2OS cell line used to study the role of the JMJD6 enzyme in DNA maintenance following DNA damage
  • Application: Western blot, Immunofluorescence
  • Production details: Gene inactivation by CRISPR-Cas9 and reintroduction of a mutant form of JMJD6 defective for its catalytic activity. Stable expression via a plasmid coding for JMJD6 mutant (H187A-D189A-H273A).
  • Biosafety level: 1
  • Recommended controls: Parental U2OS cell line, JMJD6 WT cell lines, JMJD6 deficient U2OS cell line

Target Details

  • Target: Catalytically inactive JMJD6
  • Target background: JMJD6 is an enzyme with intrinsic tyrosin kinase activity involved in the hydroxylation and de-methylation of histone and non-histone peptides with implications in the epigenetic changes of cancer. The jmjd6 gene also regulates its own splicing with the production of alternative splicied isoforms with different nuclear targets.


  • Application: Western blot, Immunofluorescence


  • Growth medium: DMEM medium supplemented with 10% foetal calf serum, sodium pyruvate and antibiotics (penicillin/streptomycin)
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Cultured in antibiotics: Penicillin, Streptomycin

Related Tools

  • Related tools: JMJD6 deficient U2OS cell line, V5-tagged form of JMJD6 U2OS cell line


  • Fages et al. 2020. PLoS Genet 16(6):e e1008511. PMID: 32598339.