#151756

C32 Colorectal adenocarcinoma Cell Line

Cat. #151756

C32 Colorectal adenocarcinoma Cell Line

Cat. #: 151756

Unit size: 1x10^6 cells / vial

Availability: 10-12 weeks

Organism: Human

Tissue: Colon

Model: Tumour line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Walter Bodmer

Institute: University of Oxford

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: C32 Colorectal adenocarcinoma Cell Line
  • Research fields: Cancer;Drug development
  • Organism: Human
  • Tissue: Colon
  • Model: Tumour line
  • Conditional: Yes
  • Description: The C32 cell line was established from a 66-year old female patient with a well differentiated adenocarcinoma of the colon classified as Dukes' stage C.
  • Cellosaurus id: CVCL_5246

Handling

  • Format: Frozen
  • Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. C32 cells grow very slowly; following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.

References

  • Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.
  • Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.