Cat. #160636
BCH-RB35 Retinoblastoma cell line
Cat. #: 160636
Sub-type: Primary
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Lateral collateral ligament (LCL)
Disease: Cancer
Model: BCOR mutation present
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Carmel McConville
Institute: University of Birmingham
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: BCH-RB35 Retinoblastoma cell line
- Cancer: Eye cancer
- Cancers detailed: Retinoblastoma
- Research fields: Cancer
- Tool sub type: Primary
- Organism: Human
- Gender: Female
- Tissue: Lateral collateral ligament (LCL)
- Disease: Cancer
- Model: BCOR mutation present
- Description: Retinoblastoma is a rare type of eye cancer most commonly affecting children under the age of 5. One eye can be affected, which is called unilateral retinoblastoma. Bilateral retinoblastoma is when both eyes are affected. This can be very distressing and frightening for the child and their parents.Some children are born with a change mutation in the RB1 retinoblastoma gene inherited from one of their parents. Or this gene change happened during the very early stages of their development in the womb. About 4 out of 10 children diagnosed (about 40%) have this heritable type, which often affects both eyes. Retinoblastoma tends to develop in just one eye in children who do not have this heritable type.We don't know what causes the remaining 60% of retinoblastomas. But these non inherited types nearly always only affect one eye.https://www.cancerresearchuk.org/about-cancer/childrens-cancer/eye-cancer-retinoblastoma/about
- Additional notes: STR data available on request
Applications
- Application notes: Tissue used: lateral collateral ligament (LCL)Patient sex: FemaleBCOR mutation presentSTR data available on request
Handling
- Format: Frozen
- Growth medium: Culture conditions: DME/F12 + 15% fetal calf serum + glutamine + non-essential amino acids + beta-mercaptoethanol - dilute fresh stock of b-ME and add just before use (Carmel needs to check concentrations). Cell line is very slow growing and should only be split at high density (e.g. 2x 25cm flasks -> 3 flasks). Before splitting, shake flask gently to dislodge apoptotic cells from surface of clumps, allow clumps to settle, remove medium/cell debris and replace with fresh medium. Do not comp...
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
