16E6/E7 HCK cell line

Expresses cervical epithelia cell markers; responds to inflammatory agents; supports growth of cervical pathogens

Contributors

Inventor Institute
Aloysius Klingelhutz The University of Iowa
Cat. #: 154442
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer
Organism: Human
Tissue: Cervix
Growth properties: E6/E7-immortalized cells generally showed an increase in telomere length as they were passaged in culture, with some later passage lines having telomeres that were similar to or longer than the earliest-passage precrisis cells examined.
Product description: Expresses cervical epithelia cell markers; responds to inflammatory agents; supports growth of cervical pathogens. Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies.
Production details: The HPV16 E6/E7 open reading frames were cloned into a murine-based retroviral vector, LXSN, which contains the neomycin resistance gene. The constructs were transfected into the packaging celline PA317, and recombinant retroviruses in the supernatant were collected. Early-passage cx cells were infected at a high titer with the different retroviral constructs, plated at various densities, selected in G418, and then passaged as pools or ring cloned. Eight ring clones were isolated for each gro…
Parental cell line: Human ectocervix cells
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: GIBCO keratinocyte serum-free media. G418 selection.
Mycoplasma free: Yes
Biosafety level: 1
References:

Klingelhutz et al. 1994. Mol Cell Biol. 14(2):961-9. PMID: 8289836.

Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells.

Related Tools for Cell lines

Cat. # Tool Name
151479 HCT 116 p300 KO [D10] Cell Line Key Info View Tool
151579 U-2 OS Gal4-p300 Cell Line Key Info View Tool
151629 MEF Raf1 FF/FF KI Cell Line Key Info View Tool
151663 MEF PKCe KO KI Cell Line Key Info View Tool
151665 MEF PKCe KO Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.