Cat. #151312
Anti-TIGAR [9C10]
Cat. #: 151312
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: TIGAR
Class: Monoclonal
Application: ChIP ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Karen Vousden
Institute: Cancer Research UK, Glasgow: The Beatson Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-TIGAR [9C10]
- Research fields: Apoptosis and autophagy;Cancer;Genetics
- Clone: 9C10
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 30 kDa
- Reactivity: Human
- Host: Mouse
- Application: ChIP ; IP ; WB
- Description: TIGAR (TP53 induced glycolysis and apoptosis regulator) is a novel p53-inducible gene, that shows similarity to the bisphosphatase domain of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, the main regulator of glycolysis. TIGAR functions to lower fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis, and an overall decrease in intracellular reactive oxygen species (ROS) levels, correlating with a decreased sensitivity to apoptosis.
- Immunogen: A 15-amino acid peptide corresponding to the exon COOH-terminal region of human TIGAR protein (CMNLQDHLNGLTETR).
- Isotype: IgG2b
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: TIGAR
- Molecular weight: 30 kDa
- Target background: TIGAR (TP53 induced glycolysis and apoptosis regulator) is a novel p53-inducible gene, that shows similarity to the bisphosphatase domain of the biFn enzyme 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, the main regulator of glycolysis. TIGAR functions to lower fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis, and an overall decrease in intracellular reactive oxygen species (ROS) levels, correlating with a decreased sensitivity to apoptosis.
Applications
- Application: ChIP ; IP ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
References
- Bensaad et al. 2006. Cell. 126(1):107-20. PMID: 16839880.
- TIGAR, a p53-inducible regulator of glycolysis and apoptosis.
