Anti-TIGAR [9C10]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-TIGAR [9C10]
• Research fields: Apoptosis and autophagy;Cancer;Genetics
• Clone: 9C10
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 30 kDa
• Reactivity: Human
• Host: Mouse
• Application: ChIP ; IP ; WB
• Description: TIGAR (TP53 induced glycolysis and apoptosis regulator) is a novel p53-inducible gene, that shows similarity to the bisphosphatase domain of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, the main regulator of glycolysis. TIGAR functions to lower fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis, and an overall decrease in intracellular reactive oxygen species (ROS) levels, correlating with a decreased sensitivity to apoptosis.
• Immunogen: A 15-amino acid peptide corresponding to the exon COOH-terminal region of human TIGAR protein (CMNLQDHLNGLTETR).
• Isotype: IgG2b
• Myeloma used: Sp2/0-Ag14

Target Details

• Target: TIGAR
• Molecular weight: 30 kDa
• Target background: TIGAR (TP53 induced glycolysis and apoptosis regulator) is a novel p53-inducible gene, that shows similarity to the bisphosphatase domain of the biFn enzyme 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, the main regulator of glycolysis. TIGAR functions to lower fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis, and an overall decrease in intracellular reactive oxygen species (ROS) levels, correlating with a decreased sensitivity to apoptosis.

Applications

• Application: ChIP ; IP ; WB

Handling

• Format: Liquid
• Concentration: 0.9-1.1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Bensaad et al. 2006. Cell. 126(1):107-20. PMID: 16839880.
• TIGAR, a p53-inducible regulator of glycolysis and apoptosis.