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#151785

Anti-Tdp2 [Tdp2]

Cat. #151785

Anti-Tdp2 [Tdp2]

Cat. #: 151785

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 10-12 weeks

Target: Tdp2

Class: Polyclonal

Application: WB

Reactivity: Human

Host: Rabbit

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Ian Waddell

Institute: Cancer Research UK, Manchester Institute

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-Tdp2 [Tdp2]
  • Research fields: Cancer;Genetics
  • Clone: Tdp2
  • Tool sub type: Primary antibody
  • Class: Polyclonal
  • Conjugation: Unconjugated
  • Molecular weight: 49 kDa
  • Reactivity: Human
  • Host: Rabbit
  • Application: WB
  • Description: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
  • Immunogen: Recombinant human protein with his tag
  • Recommended controls: A549

Target Details

  • Target: Tdp2
  • Molecular weight: 49 kDa
  • Tissue cell line specificity: A549
  • Target background: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.

Applications

  • Application: WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Unit size: 100 ug
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Dry ice

References

  • Thomson et al. 2013. Anal Biochem. 436(2):145-50. PMID: 23416181.
  • Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.

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