Anti-SDelta3 [7G12]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-SDelta3 [7G12]
• Research fields: Microbiology
• Clone: 7G12
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Virus
• Host: Mouse
• Application: IP ; WB
• Description: Monoclonal antibody used to aid understanding the method of SARS membrane fusion and entry. Severe acute respiratory syndrome coronavirus (SARS-CoV) is a species of coronavirus known to infect humans, bats, and certain other mammals. The SARS-related coronavirus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by the ACE2 receptor. SARS genome encodes for four structural proteins, namely nucleocaspid (N), spike (S), membrane (M) and envelope (E). The S protein, which forms morphologically characteristic projections on the virion surface, is responsible for binding to cellular receptor and mediating the fusion between viral and cellular membranes. The S?3 is a fragment containing residues from 168 to 461 of the S protein. This antibody has use in investigating the mechanism of membrane fusion and virus entry.
• Immunogen: GST-S?3 fusion protein
• Isotype: IgG1
• Myeloma used: Sp2/0-Ag14

Target Details

• Target: S protein of the SARS coronavirus
• Target background: Monoclonal antibody used to aid understanding the method of SARS membrane fusion and entry. Background and Research Application Severe acute respiratory syndrome coronavirus (SARS-CoV) is a species of coronavirus known to infect humans, bats, and certain other mammals. The SARS-related coronavirus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by the ACE2 receptor. SARS genome encodes for four structural proteins, namely nucleocaspid (N), spike (S), membrane (M) and envelope (E). The S protein, which forms morphologically characteristic projections on the virion surface, is responsible for binding to cellular receptor and mediating the fusion between viral and cellular membranes. The SΔ3 is a fragment containing residues from 168 to 461 of the S protein. This antibody has use in investigating the mechanism of membrane fusion and virus entry.

Applications

• Application: IP ; WB
• Application notes: Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody was found to have no viral neutralizing activity (Lip et al., 2006). Concentration 1mg/ml as standard

Handling

• Format: Liquid
• Concentration: 0.9-1.1mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Dry ice

References

• Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
• Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
• Monoclonal antibodies targeting the HR2 domain and the region immediately upstream of the HR2 of the S protein neutralize in vitro infection of severe acute respiratory syndrome coronavirus.