Cat. #152651
Anti-SDelta3 [7G12]
Cat. #: 152651
Unit size: 100 ug
Availability: 3-4 weeks
Target: S protein of the SARS coronavirus
Class: Monoclonal
Application: IP ; WB
Reactivity: Virus
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: A*STAR Accelerate Technologies Pte Ltd
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-SDelta3 [7G12]
- Research fields: Microbiology
- Clone: 7G12
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Virus
- Host: Mouse
- Application: IP ; WB
- Description: Monoclonal antibody used to aid understanding the method of SARS membrane fusion and entry. Severe acute respiratory syndrome coronavirus (SARS-CoV) is a species of coronavirus known to infect humans, bats, and certain other mammals. The SARS-related coronavirus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by the ACE2 receptor. SARS genome encodes for four structural proteins, namely nucleocaspid (N), spike (S), membrane (M) and envelope (E). The S protein, which forms morphologically characteristic projections on the virion surface, is responsible for binding to cellular receptor and mediating the fusion between viral and cellular membranes. The S?3 is a fragment containing residues from 168 to 461 of the S protein. This antibody has use in investigating the mechanism of membrane fusion and virus entry.
- Immunogen: GST-S?3 fusion protein
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: S protein of the SARS coronavirus
- Target background: Monoclonal antibody used to aid understanding the method of SARS membrane fusion and entry. Background and Research Application Severe acute respiratory syndrome coronavirus (SARS-CoV) is a species of coronavirus known to infect humans, bats, and certain other mammals. The SARS-related coronavirus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by the ACE2 receptor. SARS genome encodes for four structural proteins, namely nucleocaspid (N), spike (S), membrane (M) and envelope (E). The S protein, which forms morphologically characteristic projections on the virion surface, is responsible for binding to cellular receptor and mediating the fusion between viral and cellular membranes. The SÄÂĂÂ3 is a fragment containing residues from 168 to 461 of the S protein. This antibody has use in investigating the mechanism of membrane fusion and virus entry.
Applications
- Application: IP ; WB
- Application notes: Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody was found to have no viral neutralizing activity (Lip et al., 2006). Concentration 1mg/ml as standard
Handling
- Format: Liquid
- Concentration: 0.9-1.1mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
- Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
- Monoclonal antibodies targeting the HR2 domain and the region immediately upstream of the HR2 of the S protein neutralize in vitro infection of severe acute respiratory syndrome coronavirus.