#151855

Anti-RSV F Glycoprotein [11-5-G9]

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Cat. #151855

Anti-RSV F Glycoprotein [11-5-G9]

Cat. #: 151855

Unit size: 100 ug

Target: Human Respiratory Syncytial (RS) virus Fusion glycoprotein

Class: Monoclonal

Application: ELISA ; IF ; Fn ; WB

Reactivity: Virus

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Ayham Alnabulsi

Institute: Vertebrate Antibodies Limited

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-RSV F Glycoprotein [11-5-G9]
  • Alternate name: VP7, F
  • Research fields: Microbiology
  • Clone: 11-5-G9
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Strain: Balb/c
  • Reactivity: Virus
  • Host: Mouse
  • Application: ELISA ; IF ; Fn ; WB
  • Description: Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa.
  • Immunogen: Gradient-purified RSF-44 virus (subgroup A) UV inactivated for 20 minutes at 20C
  • Isotype: IgG1 kappa
  • Myeloma used: P3X63Ag8.653
  • Recommended controls: Immunoblot: Ag: gradient-purified RS virus (see figure). Indirect immunofluorescence: staining of RSA-2 infected BSC-1 cells

Target Details

  • Target: Human Respiratory Syncytial (RS) virus Fusion glycoprotein
  • Target background: Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa.

Applications

  • Application: ELISA ; IF ; Fn ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1mg/ml
  • Unit size: 100 ug
  • Storage buffer: Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Gimenez et al. 1986. Journal General Virology, 67: 863-870. PMID: 3517224 Gimenez et al. 1984. Journal General Virology, 65: 963-71. PMID: 6202832 Gimenez et al. 1987. Journal General Virology, 68: 1267-75. PMID: 3572364 Gimenez et al. 1996. Clinical and Diagnostic Laboratory Immunology, 3: 280-86, PMID: 8705669