Anti-Rad51C [Rad51C 2H11/6]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-Rad51C [Rad51C 2H11/6]
• Research fields: Genetics
• Clone: Rad51C 2H11/6
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Strain: Balb/c
• Host: Mouse
• Application: IF ; IHC ; IP ; WB
• Description: RAD51C is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51C is also a component for the homologous recombination pathway.
• Immunogen: Rad51C 2H11/6 was raised against His-tagged human Rad51C, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page
• Isotype: IgG1
• Myeloma used: Sp2/0-Ag14

Target Details

• Target: Rad51C
• Target background: RAD51C is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51C is also a component for the homologous recombination pathway.

Applications

• Application: IF ; IHC ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Dry ice

References

• Park et al. 2014. Oncogene. 33(40):4803-12. PMID: 24141787.
• Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair.
• French et al. 2002. J Biol Chem. 277(22):19322-30. PMID: 11912211.
• Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
• Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
• Identification and purification of two distinct complexes containing the five RAD51 paralogs.
• Masson et al. 2001. Proc Natl Acad Sci U S A. 98(15):8440-6. PMID: 11459987.
• Complex formation by the human RAD51C and XRCC3 recombination repair proteins.
• Dosanjh et al. 1998. Nucleic Acids Res. 26(5):1179-84. PMID: 9469824.
• Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.