#151309

Anti-Phospho MDM2 (Ser186) [2G2 2.2]

Cat. #151309

Anti-Phospho MDM2 (Ser186) [2G2 2.2]

Cat. #: 151309

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Murine Double Minute 2 protein (MDM2), Phosphorylated (Serine 186)

Class: Monoclonal

Application: WB ; IP ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: David Meek

Institute: University of Dundee

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Phospho MDM2 (Ser186) [2G2 2.2]
  • Research fields: Cancer;Cell biology;Genetics
  • Clone: 2G2 2.2
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 90 kDa
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: WB ; IP ; WB
  • Description: Monoclonal antibody which targets the critical ser186 residue for MDM2 function.
  • Immunogen: Peptide QRKRHKS(p)DSIS. S(p) represent a phosphorylated serine. This peptide represents the portion of MDM2 around Serine 186
  • Immunogen uniprot id: Q00987
  • Isotype: IgG1
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: Breast carcinoma

Target Details

  • Target: Murine Double Minute 2 protein (MDM2), Phosphorylated (Serine 186)
  • Molecular weight: 90 kDa
  • Tissue cell line specificity: Breast carcinoma
  • Target background: MDM2 is a ubiquitin ligase whose principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This promotes progression through the cell cycle and cell survival. MDM2 has been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumour suppressor function. MDM2 is over-expressed in many tumours, including soft tissue carcinomas and breast cancer. The phosphorylation of serines 166 and 186 is necessary for MDM2's translocation into the nucleus, thereby facilitating interaction with p53. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumour and normal tissues. This antibody is directed against serine 186, which is crucial for MDM2 function.

Applications

  • Application: WB ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

References

  • Zhang et al. 2019. J Cell Physiol. 234(7):11330-11347. PMID: 30478915.
  • Wei et al. 2013. Oncogene. 32(9):1110-20. PMID: 22525275.
  • Lenalidomide promotes p53 degradation by inhibiting MDM2 auto-ubiquitination in myelodysplastic syndrome with chromosome 5q deletion.