Anti-PHD1 [PHD112/G7]

PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha.

Contributors

Inventor Institute
Helen Turley University of Oxford
Cat. #: 151313
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer;Genetics;Metabolism;Tissue-specific biology
Application: IHC ; WB
Target: Prolyl Hydroxylase 1 (PHD1)
Reactivity: Human
Clone: PHD112/G7
Host: Mouse
Class: Monoclonal
Product description: PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation.
Conjugation: Unconjugated
Isotype: IgM
Molecular weight: 43.6 kDa
Immunogen: Full length recombinant human PHD1
Myeloma used: P3/NS1/1-Ag4.1
Target background: PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Andersen et al. 2011. PLoS One. 6(8):e23847. PMID: 21887331.

Overexpression of the HIF hydroxylases PHD1, PHD2, PHD3 and FIH are individually and collectively unfavorable prognosticators for NSCLC survival.

Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198.

Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues.

Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861.

Appelhoff et al. 2004. J Biol Chem. 279(37):38458-65. PMID: 15247232.

Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected].

Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.

Images

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Adapted from Andersen et al. 2011. PLoS One. 6(8)

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