#151313

Anti-PHD1 [PHD112/G7]

Cat. #151313

Anti-PHD1 [PHD112/G7]

Cat. #: 151313

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Prolyl Hydroxylase 1 (PHD1)

Class: Monoclonal

Application: IHC ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Helen Turley

Institute: University of Oxford

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-PHD1 [PHD112/G7]
  • Research fields: Cancer;Genetics;Metabolism;Tissue-specific biology
  • Clone: PHD112/G7
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 43.6 kDa
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; WB
  • Description: PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation.
  • Immunogen: Full length recombinant human PHD1
  • Isotype: IgM
  • Myeloma used: P3/NS1/1-Ag4.1
  • Recommended controls: MCF7 cells

Target Details

  • Target: Prolyl Hydroxylase 1 (PHD1)
  • Molecular weight: 43.6 kDa
  • Tissue cell line specificity: MCF7 cells
  • Target background: PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation.

Applications

  • Application: IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

Related Tools

  • Related tools: Anti-PHD2 [366G/76/3] ; Anti-PHD3 [EG188e/d5]

References

  • Andersen et al. 2011. PLoS One. 6(8):e23847. PMID: 21887331.
  • Overexpression of the HIF hydroxylases PHD1, PHD2, PHD3 and FIH are individually and collectively unfavorable prognosticators for NSCLC survival.
  • Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198.
  • Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues.
  • Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861.
  • Appelhoff et al. 2004. J Biol Chem. 279(37):38458-65. PMID: 15247232.
  • Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected].
  • Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.