Anti-p53 [Pab 240]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-p53 [Pab 240]
• Alternate name: Tumor Protein P53; Phosphoprotein P53; Antigen NY-CO-13; P53; Transformation-Related Protein 53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome; P53 Tumor Suppressor; Tumor Suppressor P53; TRP53; BCC7; LFS1
• Cancers detailed: Broadly Applicable
• Research fields: Apoptosis and autophagy;Cancer;Cell biology;Genetics
• Clone: Pab 240
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 53 kDa
• Strain: Balb/c
• Reactivity: Chicken ; Mammalian
• Host: Mouse
• Disease: Breast Cancer
• Application: FACS ; IHC ; IF ; IP ; WB
• Description: This monoclonal antibody reacts specifically with mutated p53 at an epitope conserved across species and normally hidden within the protein structure in non-mutant forms. Anti-p53 Pab 240 was created further understand the biochemical role of p53 in cancers and the precise effects of mutation. Anti-p53 (Pab 240) antibody recognises an evolutionarily conserved epitope upon p53 hidden within the normal protein structure, however, it does not bind to immunoprecipitated wild-type p53. This antibo...
• Immunogen: p53-b-galactosidase fusion protein containing p53 sequence from amino acids 14-389 (derived from the pSV53C p53 cDNA clone)
• Immunogen uniprot id: P04637
• Isotype: IgG1 kappa
• Myeloma used: Sp2/0-Ag14
• Recommended controls: MDA-MB-231 cell line

Target Details

• Target: p53
• Molecular weight: 53 kDa
• Tissue cell line specificity: MDA-MB-231 cell line
• Target background: p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. p53 is the most common genetic mutational event so far identified in human cancers, with the gene present on the short arm of chromosome 17 a frequent site of allele loss in common cancers. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory doma...

Applications

• Application: FACS ; IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

References

• Renzi et al. 2019. PLoS One. 14(4):e0215621. PMID: 30998743.
• Diaz Osterman et al. 2019. Elife. 8:. PMID: 31478830.
• Fekry et al. 2018. Nat Commun. 9(1):4149. PMID: 30297838.
• Katz et al. 2018. Genes Dev. 32(5-6):430-447. PMID: 29549180.
• Chen et al. 2017. Br J Pharmacol. 174(23):4345-4361. PMID: 28910492.
• Ilhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.
• Jakobsen et al. 2015. J Extracell Vesicles. 4:26659. PMID: 25735706.
• Pan et al. 2009. EMBO J. 28(22)...