Cat. #151867
Anti-NSE [NSEP2]
Cat. #: 151867
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Gamma-isozyme of human enolase (NSE)
Class: Monoclonal
Application: ELISA ; IHC ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ayham Alnabulsi
Institute: Vertebrate Antibodies Limited
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-NSE [NSEP2]
- Research fields: Cancer;Cell biology;Developmental biology;Metabolism;Neurobiology;Stem cell biology
- Clone: NSEP2
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; IHC ; WB
- Description: Neuron specific enolase (NSE, or ?-isozyme of enolase) is found at elevated concentrations in plasma in certain neoplasias, including paediatric neuroblastoma and small cell lung cancer.
- Immunogen: Immunogen: ovalbumin-conjugated synthetic peptides corresponding to human NSE amino acid sequence:NSE-P2: aa's 271-285 - TGDQLGALYQDFVRD
- Isotype: IgG1 kappa
- Myeloma used: P3X63Ag8.653
- Recommended controls: Formalin-fixed, paraffin-embedded nerve tissue sectionswestern blot: ?-isozyme of human enolase; 50-100 ng per lane
Target Details
- Target: Gamma-isozyme of human enolase (NSE)
- Tissue cell line specificity: Formalin-fixed, paraffin-embedded nerve tissue sectionswestern blot: ?-isozyme of human enolase; 50-100 ng per lane
- Target background: Neuron specific enolase (NSE, or ?-isozyme of enolase) is found at elevated concentrations in plasma in certain neoplasias, including paediatric neuroblastoma and small cell lung cancer.
Applications
- Application: ELISA ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-NSE [NSEP1]
References
- Murray et al. 1993. J Clin Pathol. 46(11):993-6. PMID: 8254105.
- Immunohistochemistry of neurone specific enolase with gamma subunit specific anti-peptide monoclonal antibodies.
- Duncan et al. 1992. J Immunol Methods. 151(1-2):227-36. PMID: 1629611.
- A simple enzyme-linked immunosorbent assay (ELISA) for the neuron-specific gamma isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences.
