Cat. #151093
Anti-Integrin b3 [F11]
Cat. #: 151093
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Integrin ?3 (CD61)
Class: Monoclonal
Application: FACS ; IHC ; IP ; Fn
Reactivity: Human ; Rat
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Mike Horton
Institute: Queen Mary University of London
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Integrin b3 [F11]
- Alternate name: Neural Cell Adhesion Molecule; NCAM; CD56 Antigen; MSK39
- Research fields: Cell biology;Tissue-specific biology;Cell signaling and signal transduction
- Clone: F11
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 180 kDa, 140 kDa, 120 kDa
- Strain: Balb/c
- Reactivity: Human ; Rat
- Host: Mouse
- Application: FACS ; IHC ; IP ; Fn
- Description: The F11 epitope is different to the MHF4 epitope.
- Immunogen: A bone cell suspension containing osteoclasts, osteoblasts and bone marrow cells obtained from the long bones of newborn Sprague Dawley rats.
- Isotype: IgG1
- Myeloma used: P3X63Ag8.653
- Recommended controls: Neuroblastome
Target Details
- Target: Integrin ?3 (CD61)
- Molecular weight: 180 kDa, 140 kDa, 120 kDa
- Tissue cell line specificity: Neuroblastome
- Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin b3 is expressed in osteoclasts, megacaryocytes and platelets.
Applications
- Application: FACS ; IHC ; IP ; Fn
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- de Nigris et al. 2007. Oncogene. 26(3):382-94. PMID: 16878156.
- Cooperation between Myc and YY1 provides novel silencing transcriptional targets of alpha3beta1-integrin in tumour cells.
- Thompson et al. 2000. J Immunol. 165(1):426-34. PMID: 10861081.
- Divergent effects of platelet-endothelial cell adhesion molecule-1 and beta 3 integrin blockade on leukocyte transmigration in vivo.
- Shankar et al. 1993. J Cell Sci. 105 ( Pt 1):61-8. PMID: 7689577.
- Integrin receptor-mediated mobilisation of intranuclear calcium in rat osteoclasts.
- Helfrich et al. 1992. J Bone Miner Res. 7(3):345-51. PMID: 1585837.
- Helfrich et al. 1992. J Bone Miner Res. 7(3):335-43. PMID: 1585836.
- Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3.
- Rat osteoclasts adhere to a wide range of RGD (Arg-Gly-Asp) peptide-containing proteins, including the bone sialoproteins and fibronectin, via a beta 3 integrin.
