#161656

Anti-integrin a3 inhibiting

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Cat. #161656

Anti-integrin a3 inhibiting

Cat. #: 161656

Sub-type: Primary antibody

Target: Integrin alpha-3 (CD49b)

Class: Monoclonal

Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Elizabeth A. Wayner, William Carter

Institute: Fred Hutchinson Cancer Research Center

Primary Citation: Wayner et al.1987. Journal of Cell Biology.105(4):1873-84. PMID: 2822727

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-integrin a3 inhibiting
  • Research fields: Cell biology
  • Clone: P1B5
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Molecular weight: 119kDa
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
  • Description: Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. Integrin ?3 (P1B5) is a mouse monoclonal antibody developed to identify four distinct classes (I, II, III, IV) of cell surface receptors for native collagen. P1B5 inhibited attachment to type VI and I collagens and partially inhibited attachment to fibronectin and laminin as well.
  • Immunogen: HT1080 human fibrosarcoma cell line
  • Isotype: IgG1

Target Details

  • Target: Integrin alpha-3 (CD49b)
  • Molecular weight: 119kDa
  • Target background: ?2?1 complex of Collagen receptors: I, II, III, IV. P1B5 inhibits cell adhesion to collagen and fibronectin.

Applications

  • Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

  • Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

References

  • Wayner et al.1987. Journal of Cell Biology.105(4):1873-84.PMID: 2822727
  • Carter et al.1990. J Cell Biol.111(6 ):3141-54.PMID: 2269668
  • Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064