Cat. #153418
Anti-HIV gp41 [SAR1]
Cat. #: 153418
Unit size: 100 ug
Availability: 10-12 weeks
Target: HIV-1 gp41 tail sequence GERDRDR
Class: Monoclonal
Application: ELISA ; Fn ; WB
Reactivity: Virus
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Nigel Dimmock
Institute: University of Warwick
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-HIV gp41 [SAR1]
- Alternate name: Env antibody, Glycoprotein 41 antibody, gp41 antibody, Human Immunodeficiency Virus 1 antibody, TM antibody, Transmembrane protein antibody
- Research fields: Immunology;Microbiology
- Clone: SAR1
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Virus
- Host: Mouse
- Application: ELISA ; Fn ; WB
- Description: The envelope protein of human immunodeficiency virus type 1 (HIV-1) is synthesized as a gp160 polypeptide that is cleaved to form a non-covalently linked heterodimer; an external gp120 domain and a transmembrane anchoring gp41 domain. The monoclonal antibody SAR1 is a novel IgG that was raised by immunising mice with a plant virus chimera expressing the gp41 tail sequence GERDRDR. SAR1 recognizes gp41 expressed on the surface of infected cells, and binds to some, but not all, preparations of purified virions, suggesting that it may recognize non-infectious virions or degraded/immature forms of the envelope protein. SAR1 actively neutralized virus after it had attached to target cells but had little activity against free virions. Data suggest that SAR1 mediates post-attachment neutralization of virus bound to target cells by inhibiting virus-mediated fusion.
- Immunogen: Mice were infected with Cowpea mosaic virus-HIV-1 gp41 chimera (30 ?g/ml) expressing the gp41 C-terminal sequence GERDRDR
- Isotype: IgG2a kappa
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: HIV-1 gp41 tail sequence GERDRDR
- Target background: The envelope protein of human immunodeficiency virus type 1 (HIV-1) is synthesized as a gp160 polypeptide that is cleaved to form a non-covalently linked heterodimer; an external gp120 domain and a transmembrane anchoring gp41 domain. The monoclonal antibody SAR1 is a novel IgG that was raised by immunising mice with a plant virus chimera expressing the gp41 tail sequence GERDRDR. SAR1 recognizes gp41 expressed on the surface of infected cells, and binds to some, but not all, preparations of purified virions, suggesting that it may recognize non-infectious virions or degraded/immature forms of the envelope protein. SAR1 actively neutralized virus after it had attached to target cells but had little activity against free virions. Data suggest that SAR1 mediates post-attachment neutralization of virus bound to target cells by inhibiting virus-mediated fusion.
Applications
- Application: ELISA ; Fn ; WB
Handling
- Format: Liquid
- Unit size: 100 ug
- Shipping conditions: Dry ice
References
- Heap et al. 2005. J Gen Virol. 86(Pt 5):1499-507. PMID: 15831963.
- Cheung et al. 2005. J Gen Virol. 86(Pt 1):131-8. PMID: 15604440.
- Reading et al. 2003. Virology. 315(2):362-72. PMID: 14585339.
