Cat. #151516
Anti-Goblet cells [FIS 3G12/3]
Cat. #: 151516
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Goblet cells in the posterior part of the zebrafish intestine
Class: Monoclonal
Application: IHC ; IF ; WB
Reactivity: Zebrafish
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Linda Ariza-McNaughton
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Goblet cells [FIS 3G12/3]
- Clone: FIS 3G12/3
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 175 kDa, 120 kDa, 70 kDa
- Strain: Balb/c
- Reactivity: Zebrafish
- Host: Mouse
- Application: IHC ; IF ; WB
- Description: 3G12 is a marker for mucus-secreting cells in the posterior gut of zebrafish.
- Immunogen: Lysate of fish gut intestine
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Goblet cells in the posterior part of the zebrafish intestine
- Molecular weight: 175 kDa, 120 kDa, 70 kDa
- Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. Antibody 3G12 marks goblet cells specifically. It labels both the mucus compartment and other parts of the cytoplasm. On Western blots, 3G12 stains diffuse bands, consistent with variably glycosylated antigen(s). 3G12 labelled cells are concentrated in the posterior gut.
Applications
- Application: IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
References
- Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
- Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.
