Anti-FBW7 [FBOX 3a9/1]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-FBW7 [FBOX 3a9/1]
• Alternate name: F-Box And WD Repeat Domain Containing 7; F-Box Protein FBX3; SEL-1; Fbx3; HCdc4; FBW7; Hago; Homolog Of C Elegans Sel-1; F-Box Protein SEL-1; Archipelago Homolog; F-Box Protein FBW7; Archipelago; FBXO3; FBXW6; CDC4; FBW6; AGO
• Research fields: Biochemistry;Cancer
• Clone: FBOX 3a9/1
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human ; Mouse
• Host: Mouse
• Application: FACS ; IHC ; IF ; IP ; WB
• Description: SCF (Skp, Cullin, F-box containing complex) ubiquitin ligases regulate the degradation of many proteins involved in the control of cell division and growth. F-box proteins are the SCF components that bind to substrates, and this binding is usually signaled by substrate phosphorylation. The FBW7/Cdc4 F-box protein was first recognized by its ability to bind cyclin E, and the SCF (FBW7) is now known to target c-Myc, c-Jun and Notch for degradation in addition to its role in cyclin E proteolysis. FBW7 thus negatively regulates several key oncoproteins. Accordingly, FBW7 is a tumor suppressor that is mutated in a wide spectrum of human cancers, and FBW7 functions as a haplo-insufficient tumor suppressor in mice.
• Immunogen: Synthetic peptide of human sequence
• Isotype: IgG1
• Myeloma used: Sp2/0-Ag14

Target Details

• Target: F-box/WD repeat-containing protein 7 (FBW7, Cdc4)
• Target background: SCF (Skp, Cullin, F-box containing complex) ubiquitin ligases regulate the degradation of many proteins involved in the control of cell division and growth. F-box proteins are the SCF components that bind to substrates, and this binding is usually signaled by substrate phosphorylation. The FBW7/Cdc4 F-box protein was first recognized by its ability to bind cyclin E, and the SCF (FBW7) is now known to target c-Myc, c-Jun and Notch for degradation in addition to its role in cyclin E proteolysis. FBW7 thus negatively regulates several key oncoproteins. Accordingly, FBW7 is a tumor suppressor that is mutated in a wide spectrum of human cancers, and FBW7 functions as a haplo-insufficient tumor suppressor in mice.

Applications

• Application: FACS ; IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Dry ice

References

• Tu et al. 2013. PLoS One. 8(7):e68574. PMID: 23840897.
• Recombinant human adenovirus-p53 injection induced apoptosis in hepatocellular carcinoma cell lines mediated by p53-Fbxw7 pathway, which controls c-Myc and cyclin E.
• Nateri et al. 2004. Science. 303(5662):1374-8. PMID: 14739463.
• The ubiquitin ligase SCFFbw7 antagonizes apoptotic JNK signaling.