Anti-CyclinA [E67.1]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CyclinA [E67.1]
• Alternate name: CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2Human Entrez Gene ID: 89 Human SwissProt: P2248 Human Unigene: 58974 Human Gene Symbol: CCNA2Human Chromosome Location: 4q27
• Research fields: Cancer;Cell biology;Genetics
• Clone: E67.1
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 54 kDa
• Reactivity: Bovine ; Human ; Mink ; Mouse
• Host: Mouse
• Application: FACS ; IHC ; IF ; IP ; WB
• Description: Monoclonal antibody capable of immunoprecipitating active CDK/Cyclin A H1 kinase.
• Immunogen: Full length bovine Cyclin A protein
• Immunogen uniprot id: P78396
• Isotype: IgG2a kappa
• Recommended controls: K-562 and HeLa Cells

Target Details

• Target: Cyclin A
• Molecular weight: 54 kDa
• Tissue cell line specificity: K-562 and HeLa Cells
• Target background: The anti-Cyclin A2, clone E67.1, recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located on amino acids 144-148 of human Cyclin A2. Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs), controlling transition at various specific phases of the cell cycle. Cyclin A is involved in the regulation of the cell cycle and is essential for progression through the S-phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S-phase, with passage through to the G2 phase. E67.1 is one of the only antibodies capable of immunoprecipitating active CDK/Cyclin A H1 kinase from such a wide range of species. This antibody binds to an epitope within amino acids 144-148 of human Cyclin A2, mapped out by phage selection.

Applications

• Application: FACS ; IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

References

• Jones et al. 2018. J Cell Biol. 217(9):3203-3218. PMID: 29930204.
• Roilo et al. 2018. Nucleic Acids Res. 46(6):3198-3210. PMID: 29361038.
• Whalley et al. 2015. Nat Commun. 6:7437. PMID: 26078008.
• Ge et al. 2010. J Cell Biol. 191(7):1285-97. PMID: 21173116.
• Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories.
• Ho et al. 2008. J Biol Chem. 283(9):5477-85. PMID: 18077453.
• Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network.
• Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
• Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
• Sandhu et al. 2000. Oncogene. 19(47):5314-23. PMID: 11103932.
• Reduction of Cdc25A contributes to cyclin E1-Cdk2 inhibition at senescence in human mammary epithelial cells.
• Slingerland et al. 1994. Mol Cell Biol. 14(6):3683-94. PMID: 8196612.
• A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells.
• Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
• Simian virus 40 large T antigen associates with cyclin A and p33cdk2.