#151704

Anti-CLEC9A [397]

Cat. #151704

Anti-CLEC9A [397]

Cat. #: 151704

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Mouse DNGR1

Class: Monoclonal

Application: WB

Reactivity: Mouse

Host: Rat

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-CLEC9A [397]
  • Alternate name: C-Type Lectin Domain Family 9 Member A; HEEE9341; UNQ9341; DNGR-1; CD37; DNGR1
  • Research fields: Immunology;Stem cell biology
  • Clone: 397
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: Mouse
  • Host: Rat
  • Application: WB
  • Description: Recognises mouse DNGR-1, also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognise a preformed signal exposed on necrotic cells.
  • Immunogen: RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope
  • Isotype: IgG2a
  • Myeloma used: Y3.AG.1.2.3
  • Recommended controls: rat IgG2a isotype-matched control mAbs

Target Details

  • Target: Mouse DNGR1
  • Tissue cell line specificity: rat IgG2a isotype-matched control mAbs
  • Target background: Recognises mouse DNGR-1, also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognise a preformed signal exposed on necrotic cells.

Applications

  • Application: WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Joffre et al. 2010. Eur J Immunol. 40(5):1255-65. PMID: 20333625.
  • Efficient and versatile manipulation of the peripheral CD4+ T-cell compartment by antigen targeting to DNGR-1/CLEC9A.
  • Sancho et al. 2009. Nature. 458(7240):899-903. PMID: 19219027.
  • Identification of a dendritic cell receptor that couples sensing of necrosis to immunity.
  • Sancho et al. 2008. J Clin Invest. 118(6):2098-110. PMID: 18497879.
  • Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin.