Cat. #153780
Anti-CEP164 [1F3G10]
Cat. #: 153780
Sub-type: Primary antibody
Unit size: 100 ug
Target: Anti-CEP164 [1F3G10]
Class: Monoclonal
Application: IHC ; IF ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ciaran Morrison
Institute: National University of Ireland, Galway; Centre For Chromosome Biology
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CEP164 [1F3G10]
- Alternate name: Centrosomal protein of 164 kDa, KIAA152, NPHP15
- Research fields: Cancer;Cell biology;Metabolism
- Clone: [1F3G10]
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 164 kDa (runs c.190)
- Reactivity: Human
- Host: Mouse
- Application: IHC ; IF ; IP ; WB
- Description: CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1.
- Immunogen: Recombinant human CEP164 amino acids 6-296 (bacterially expressed from pGEX4T as a GST fusion, then thrombin-cleaved and purified).
- Isotype: IgG1 kappa
- Myeloma used: Sp2/0-Ag14
- Recommended controls: hTERT-RPE1 Cell Line
Target Details
- Target: Anti-CEP164 [1F3G10]
- Molecular weight: 164 kDa (runs c.190)
- Tissue cell line specificity: hTERT-RPE1 Cell Line
- Target background: CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1.
Applications
- Application: IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Flynn et al. 2020. Hum Mol Genet. 29(3):407-417. PMID: 31868218.
- Khouj et al. 2019. J Cell Sci. 132(19):. PMID: 31492759.
- Ogungbenro et al. 2018. J Cell Biol. 217(4):1205-1215. PMID: 29440264.
- Sieben et al. 2018. Nat Methods. 15(10):777-780. PMID: 30275574.
- Daly et al. 2016. J Cell Sci. 129(9):1769-74. PMID: 26966185.
