Skip to main content

#151310

Anti-Cdc20 [AR12.4]

Cat. #151310

Anti-Cdc20 [AR12.4]

Cat. #: 151310

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Cell division cycle protein 20 (cdc20)

Class: Monoclonal

Application: FACS ; IHC ; IF ; IP

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: European Institute of Oncology

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-Cdc20 [AR12.4]
  • Alternate name: Fizzy protein, CDC2, CDC2A, p55CDC, P55CDC-LSBHuman Entrez Gene ID 991 Human SwissProt Q12834 Human Unigene 524947 Human Gene Symbol CDC2Human Chromosome Location 1p34.2
  • Research fields: Biochemistry;Cell biology
  • Clone: AR12.4
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 55 kDa
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP
  • Description: Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
  • Immunogen: Urea-denatured His6 human Cdc20 recombinant protein
  • Isotype: IgG1 kappa
  • Myeloma used: P3X63Ag8.653
  • Recommended controls: Ramos or HeLa cells. Tonsil or gastric carcinoma.

Target Details

  • Target: Cell division cycle protein 20 (cdc20)
  • Molecular weight: 55 kDa
  • Tissue cell line specificity: Ramos or HeLa cells. Tonsil or gastric carcinoma.
  • Target background: Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.

Applications

  • Application: FACS ; IHC ; IF ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Dry ice

References

  • Sironi et al. 2001. EMBO J. 20(22):6371-82. PMID: 11707408.
  • Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint.

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.