Anti-CD68 [KP1]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD68 [KP1]
• Alternate name: CD68 Molecule; CD68 Antigen; Macrophage Antigen CD68; GP11; Scavenger Receptor Class D; Member; Scavenger Receptor Class D; SCARD1; LAMP4
• Research fields: Cell biology;Immunology;Stem cell biology
• Clone: KP1
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IF ; IP ; WB
• Description: Monoclonal antibody which recognises most human macrophages, useful in studying macrophage disorders including both reactive and neoplastic states.
• Immunogen: Lysosomal contents of lung macrophages
• Immunogen uniprot id: P34810
• Isotype: IgG1
• Myeloma used: P3/NS1/1-Ag4.1
• Recommended controls: Human spleen tissue, Mouse brain tissue or human liver tissue (IHC); Human lung cancer (IF)

Target Details

• Target: CD68
• Tissue cell line specificity: Human spleen tissue, Mouse brain tissue or human liver tissue (IHC); Human lung cancer (IF)
• Target background: Anti-CD68 (KP1) monoclonal antibody recognises a fixation-resistant epitope in a wide variety of tissue macrophages (such as Kupffer cells germinal centre, splenic, and lamina propria macrophages), and in granulocyte precursors. Anti-CD68 (KP1) recognises most human macrophages in routinely processed tissue, where the target can be identified by immunocytochemical techniques. CD68 is a 110-kD transmembrane glycoprotein which is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and favours binding to lectins through its heavily glycosylated extracellular domain. CD68 is also a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. CD68 is resistant to conventional fixatives such as formalin and B5, as such KP1 may be of practical importance for diagnostic pathologists. CD68 persists throughout cell maturation from the monocyte to the mature macrophage stage. The quantity of this antigen increases upon macrophages when cells are activated and mature indicated by a greater intensity of staining. KP1 is widely used to detect macrophages in paraffin embedded tissue sections.

Applications

• Application: FACS ; IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

References

• Fovet et al. 2019. EBioMedicine. 47:492-505. PMID: 31492559.
• Malissen et al. 2019. Oncoimmunology. 8(12):e1665976. PMID: 31741766.
• MacGregor et al. 2019. Oncoimmunology. 8(12):e1665460. PMID: 31741762.
• Kothari et al. 2018. Brain Pathol. 28(6):806-821. PMID: 30062819.
• Fodil et al. 2017. Nat Commun. 8(1):932. PMID: 29030607.
• Wen et al. 2017. Cell Death Dis. 8(2):e2593. PMID: 28151470.
• Adipocytes activate mitochondrial fatty acid oxidation and autophagy to promote tumor growth in colon cancer.
• Hyafil et al. 2016. J Nucl Med. :. PMID: 27789718.
• Imaging the Cytokine Receptor CXCR4 in Atherosclerotic Plaques with the Radiotracer 68Ga-Pentixafor for PET.
• Tissue factor-bearing microparticles and CA19.9: two players in pancreatic cancer-associated thrombosis?
• Woei-A-Jin et al. 2016. Br J Cancer. :. PMID: 27404454.
• Sugimoto et al. 2016. J Clin Exp Hematop. 56(1):1-19. PMID: 27334853.
• Karamese et al. 2016. Immunopharmacol Immunotoxicol. 38(3):228-37. PMID: 27144896.
• Anti-oxidant and anti-inflammatory effects of apigenin in a rat model of sepsis: an immunological, biochemical, and histopathological study.
• a4-Integrin Antibody Treatment Blocks Monocyte/Macrophage Traffic to, Vascular Cell Adhesion Molecule-1 Expression in, and Pathology of the Dorsal Root Ganglia in an SIV Macaque Model of HIV-Peripheral Neuropathy.
• Lakritz et al. 2016. Am J Pathol. :. PMID: 27157989.
• Armstrong et al. 2016. Oncotarget. 7(7):7885-98. PMID: 26799286.
• PTEN deficiency promotes macrophage infiltration and hypersensitivity of prostate cancer to IAP antagonist/radiation combination therapy.
• Follicular Lymphoma: The Role of the Tumor Microenvironment in Prognosis.
• Liu et al. 2015. Medicine (Baltimore). 94(39):e1631. PMID: 26426650.
• Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer.
• Lakritz et al. 2015. Am J Pathol. :. PMID: 25956030.
• Monocyte Traffic, Dorsal Root Ganglion Histopathology, and Loss of Intraepidermal Nerve Fiber Density in SIV Peripheral Neuropathy.
• Zhou et al. 2013. Proc Natl Acad Sci U S A. 110(46):E4335-44. PMID: 24167262.
• Fibrinogen-specific antibody induces abdominal aortic aneurysm in mice through complement lectin pathway activation.
• Chamberlain et al. 2013. PLoS One. 8(8):e71631. PMID: 23936523.
• Interleukin expression after injury and the effects of interleukin-1 receptor antagonist.
• Kunisch et al. 2004. Ann Rheum Dis. 63(7):774-84. PMID: 15194571.
• Macrophage specificity of three anti-CD68 monoclonal antibodies (KP1, EBM11, and PGM1) widely used for immunohistochemistry and flow cytometry.
• Doussis et al. 1993. J Clin Pathol. 46(4):334-6. PMID: 7684403.
• CD68 reactivity of non-macrophage derived tumours in cytological specimens.
• Pulford et al. 1990. Int Immunol. 2(10):973-80. PMID: 2078523.
• Distribution of the CD68 macrophage/myeloid associated antigen.
• Micklem et al. 1989. Br J Haematol. 73(1):6-11. PMID: 2803980.
• A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
• Pulford et al. 1989. J Clin Pathol. 42(4):414-21. PMID: 2654191.
• KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.