Anti-CD45RB [PD7/26] mAb

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD45RB [PD7/26] mAb
• Alternate name: Protein Tyrosine Phosphatase; Receptor Type C; CD45 Antigen; CD45; L-CA; T2; Protein Tyrosine Phosphatase; Receptor Type; C Polypeptide; T2 Leukocyte Common Antigen; T2 Glycoprotein; EC 3.1.3.48; GP18; CD45R; B22; LCA; LY5
• Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Immunology;Stem cell biology
• Clone: PD7/26
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IF ; WB
• Description: Monoclonal antibody capable of detecting CD45RB in paraffin-embedded samples, and aids in differentiating between lymphoid and non-lymphoid tumours of B-cell origin.
• Immunogen: Human PBL maintained in T-cell growth factor
• Immunogen uniprot id: P08575
• Isotype: IgG1
• Myeloma used: P3/NS1/1-Ag4.1

Target Details

• Target: Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RB)
• Target background: CD45RB is a protein tyrosine phosphatase receptor that is present on most hematopoietic cells and absent on non-hematopoietic cells. It is a transmembrane pan-leukocyte protein with tyrosine phosphatase activity and is involved in the regulation of signal transduction in haematopoiesis and the threshold of T cell antigen receptor (TCR) signalling. This is via dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). Expression of CD45RB is more limited than CD45 and can only be found on T cell subsets, B, NK, and myeloid cells. Regulatory T cells (Tregs) have been shown to express low levels of CD45RB, linked to increased migration to sites of infection. CD45RB is expressed on the majority of lymphomas and leukaemia's of B-cell origin. Anti-CD45RB is useful in differentiating between lymphoid and non-lymphoid tumours. CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling. All CD45 isoforms share the same transmembrane and cytoplasmic domains, however their extracellular domains vary, depending on the spliced product. Anti-CD45RB is capable of recognising antigens in fixed and paraffin-embedded tissues, unlike other reagents of similar specificity. This antibody reacts with an epitope which does not become denatured or masked during fixation and embedding.

Applications

• Application: FACS ; IHC ; IF ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

References

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