Anti-CD33 [6C5/2]

CD33 is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.

Contributors

Inventor Institute
Paul Crocker University of Oxford
Cat. #: 151203
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer;Cell biology;Immunology;Stem cell biology
Application: ELISA ; FACS ; IHC
Target: SIGLEC3 (CD33)
Reactivity: Human
Clone: CD33 6C5/2
Host: Mouse
Class: Monoclonal
Alternate name: Sialic Acid Binding Ig Like Lectin 12; SIGLECL1; SLG 3; Siglec-12; Siglec-L1; S2V
Product description: The SIGLECs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. CD33 is found on granulocyte and macrophage precursors in the bone marrow, but not on pluripotent stem cells. CD33 is also expressed on, and a useful marker for, peripheral monocytes. CD33 is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. CD33 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells & preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Conjugation: Unconjugated
Isotype: IgG1
Immunogen: CD33-Fc recombinant protein
Myeloma used: Sp2/0-Ag14
Target background: The SIGLECs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. CD33 is found on granulocyte and macrophage precursors in the bone marrow, but not on pluripotent stem cells. CD33 is also expressed on, and a useful marker for, peripheral monocytes. CD33 is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. CD33 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells & preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Souza et al. 2019. Short-Term High-Fat Diet Consumption Reduces Hypothalamic Expression of the Nicotinic Acetylcholine Receptor a7 Subunit (a7nAChR) and Affects the Anti-inflammatory Response in a Mouse Model of Sepsis Front Immunol. 10:565. PMID: 309678

Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.

The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2.

Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules.

Images

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Adapted from Zou et al. 2011. PLoS One. 6(9)

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