Anti-CD31 (PECAM1) [JC70] mAb

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD31 (PECAM1) [JC70] mAb
• Alternate name: Platelet/Endothelial Cell Adhesion Molecule 1; CD31 Antigen; EndoCAM; PECAM-1; GPIIA; PECA1; CD31/EndoCAM; CD31
• Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Stem cell biology;Tissue-specific biology
• Clone: JC70
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IF ; WB
• Description: Monoclonal antibody directed against endothelial cell marker (CD31, JC70), capable of evaluating tumour angiogenesis and angiosarcoma more effectively than anti-FVIII-Reg.
• Immunogen: Cell membrane preparation from the spleen of a patient with hairy cell leukaemia
• Immunogen uniprot id: P16284
• Isotype: IgG1
• Myeloma used: P3/NS1/1-Ag4.1

Target Details

• Target: PECAM1 (CD31)
• Target background: CD31/JC70, also known as platelet endothelium cell adhesion molecule (PECAM-1), is a 130kDa transmembrane glycoprotein found on cell surface of endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, lymphocytes (T cells, B cells, and NK cells), megakaryocytes, and osteoclasts. PECAM1 is a member of the immunoglobulin (Ig) superfamily. It is also a major constituent of the endothelial cell intercellular junction and expressed in certain cancers. PECAM1 is involved in cell signalling and adhesion, trans-endothelial migration of leukocytes, angiogenesis, and integrin activation. PECAM1 is a specific marker of endothelial differentiation. Anti-CD31 be used to identify the presence of endothelial cells in a histological tissue sample to determine the degree of angiogenesis (which in turn can determine tumour severity/growth). Anti-CD31 stains a fixation resistant epitope on endothelial cells in both benign and malignant endothelial cells in angiosarcomas with more consistency than monoclonal or polyclonal antibodies to FV111-Rag . CD31 is a good marker of endothelial cells in paraffin sections. Anti-CD31 was created to recognise membrane bound glycoprotein CD31/JC70.

Applications

• Application: FACS ; IHC ; IF ; WB

Handling

• Format: Liquid
• Concentration: 0.9-1.1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Dry ice

References

• Muller WA, Kishimoto T, et al (eds) 1997. Leucocyte Typing VI, Garland Publishing Inc., New York and London, p 362-364. ISBN-13: 978-0815327455
• Thomson et al. 2018. Methods Mol Biol. 1846:153-160. PMID: 30242758.
• Ceasrine et al. 2018. Elife. 7:. PMID: 30303066.
• Frye et al. 2018. Nat Commun. 9(1):1511. PMID: 29666442.
• Fujita et al. 2017. PLoS One. 12(9):e0184534. PMID: 28886194.
• Tissue factor-bearing microparticles and CA19.9: two players in pancreatic cancer-associated thrombosis?
• Woei-A-Jin et al. 2016. Br J Cancer. :. PMID: 27404454.
• Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.
• A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway.
• Zhao et al. 2013. Cancer Res. 73(20):6149-63. PMID: 24097821.
• Novel modeling of cancer cell signaling pathways enables systematic drug repositioning for distinct breast cancer metastases.
• Ding et al. 2013. PLoS One. 8(5):e63628. PMID: 23675495.
• HIF-1a transgenic bone marrow cells can promote tissue repair in cases of corticosteroid-induced osteonecrosis of the femoral head in rabbits.
• Orecchia et al. 2011. PLoS One. 6(9):e24307. PMID: 21931678.
• Sirtinol treatment reduces inflammation in human dermal microvascular endothelial cells.
• Parums et al. 1990. J Clin Pathol. 43(9):752-7. PMID: 2212067.
• JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections.